Tryps-IN: A streamlined palaeoproteomics workflow enables ZooMS analysis of 10,000-year-old petrous bones from Jordan rift-valley
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Tryps-IN : A streamlined palaeoproteomics workflow enables ZooMS analysis of 10,000-year-old petrous bones from Jordan rift-valley. / Jensen, Theis Zetner Trolle; Yeomans, Lisa; Le Meillour, Louise; Nielsen, Pia Wistoft; Ramsøe, Max; Mackie, Meaghan; Bangsgaard, Pernille; Kinzel, Moritz; Thuesen, Ingolf; Collins, Matthew J.; Taurozzi, Alberto J.
In: Journal of Archaeological Science: Reports, Vol. 52, 104238, 2023.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Tryps-IN
T2 - A streamlined palaeoproteomics workflow enables ZooMS analysis of 10,000-year-old petrous bones from Jordan rift-valley
AU - Jensen, Theis Zetner Trolle
AU - Yeomans, Lisa
AU - Le Meillour, Louise
AU - Nielsen, Pia Wistoft
AU - Ramsøe, Max
AU - Mackie, Meaghan
AU - Bangsgaard, Pernille
AU - Kinzel, Moritz
AU - Thuesen, Ingolf
AU - Collins, Matthew J.
AU - Taurozzi, Alberto J.
PY - 2023
Y1 - 2023
N2 - Poor preservation of collagen in dry and/or arid environments has hindered the application of Zooarchaeology by mass spectrometry (ZooMS) analysis in many regions of the world. As a result, many zooarchaeological investigations have relied exclusively on morphological assessment of fragmentary remains due to the inadequate preservation of biomolecules. The climatic conditions of Southwest Asia include extreme temperature fluctuations unconducive to preservation of proteins and DNA. We performed zooarchaeological analysis of remains from the 10,000-year-old site of Shkārat Msaied in Jordan and sub-sampled twenty-eight petrous bones, the hardest bone in the mammalian skeleton, for species identification by ZooMS. Using an unconventional and simplified extraction protocol we call Tryps-IN, in which digestion was performed without removal of the demineralising EDTA, we taxonomically identified several fragments, outperforming an established ZooMS workflow. A subset of identifications was subsequently confirmed using liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) protein sequencing. The new methodology presented here opens the possibility of further bioarchaeological investigation of other fragmentary faunal assemblages within this region of archaeological significance.
AB - Poor preservation of collagen in dry and/or arid environments has hindered the application of Zooarchaeology by mass spectrometry (ZooMS) analysis in many regions of the world. As a result, many zooarchaeological investigations have relied exclusively on morphological assessment of fragmentary remains due to the inadequate preservation of biomolecules. The climatic conditions of Southwest Asia include extreme temperature fluctuations unconducive to preservation of proteins and DNA. We performed zooarchaeological analysis of remains from the 10,000-year-old site of Shkārat Msaied in Jordan and sub-sampled twenty-eight petrous bones, the hardest bone in the mammalian skeleton, for species identification by ZooMS. Using an unconventional and simplified extraction protocol we call Tryps-IN, in which digestion was performed without removal of the demineralising EDTA, we taxonomically identified several fragments, outperforming an established ZooMS workflow. A subset of identifications was subsequently confirmed using liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) protein sequencing. The new methodology presented here opens the possibility of further bioarchaeological investigation of other fragmentary faunal assemblages within this region of archaeological significance.
U2 - 10.1016/j.jasrep.2023.104238
DO - 10.1016/j.jasrep.2023.104238
M3 - Journal article
VL - 52
JO - Journal of Archaeological Science: Reports
JF - Journal of Archaeological Science: Reports
SN - 2352-409X
M1 - 104238
ER -
ID: 370269787